• Login
    View Item 
    •   Repository Home
    • Staff Publications
    • School of Pure and Applied Sciences
    • View Item
    •   Repository Home
    • Staff Publications
    • School of Pure and Applied Sciences
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Evaluation of Loop-Mediated Isothermal Amplification Suitable for Molecular Monitoring of Schistosome-Infected Snails in Field Laboratories

    Thumbnail
    View/Open
    HamburgeretalFieldLAMPforSchistosomainfectedsnailsAJTMH2012.pdf (977.9Kb)
    Date
    2013
    Author
    Hamburger, Joseph
    Abbasi, Ibrahim
    Kariuki, Curtis
    Wanjala, Atsabina
    Mzungu, Elton
    Mungai, Peter
    Muchiri, Eric
    King, Charles H.
    Metadata
    Show full item record
    Abstract
    We previously described loop-mediated isothermal amplification (LAMP) for detection of Schistosoma haematobium and S. mansoni DNA in infected snails. In the present study, we adapted the LAMP assay for application in field laboratories in schistosomiasis-endemic areas. Isolation of DNA was simplified by blotting snail tissue (extracted in NaOH/sodium dodecyl sulfate) onto treated membranes, which enabled preservation at ambient temperatures. A ready-mix of LAMP reagents, suitable for shipment at ambient temperature and storage in minimal refrigeration, was used. Local survey teams without experience in molecular biology acquired operational expertise with this test within a few hours. Fifty-four field-caught snails were tested locally by LAMP and 59 were tested at similar conditions in Jerusalem. The LAMP results were consistent with those of a polymerase chain reaction; only four samples showed false-negative results. Results indicate that LAMP assays are suitable for detection of S. haematobium and S. mansoni in low-technology parasitology laboratories in which schistosomiasis elimination activities are undertaken.
    URI
    http://repository.must.ac.ke/handle/123456789/933
    Collections
    • School of Pure and Applied Sciences [170]

    MUST Repository copyright © 2002-2016  MUST Repository
    Contact Us | Send Feedback
    Theme by 
    MUST Repository
     

     

    Browse

    All of the RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    MUST Repository copyright © 2002-2016  MUST Repository
    Contact Us | Send Feedback
    Theme by 
    MUST Repository